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IC 50 values of standard CYP substrate inhibitors and remdesivir for CYP3A4, 2C8, and 2D6 isoenzymes.

Journal: Frontiers in Pharmacology

Article Title: In vitro effect of Withania somnifera , AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb−drug interactions in the management of COVID-19

doi: 10.3389/fphar.2022.973768

Figure Lengend Snippet: IC 50 values of standard CYP substrate inhibitors and remdesivir for CYP3A4, 2C8, and 2D6 isoenzymes.

Article Snippet: The concentration of case–control small-molecule remdesivir was prepared in a range of 1–1,000 ng/ml ( ).

Techniques:

Effect of (A) ketoconazole (5.00–0.0005 μM/ml), (B) remdesivir (1,000–1 ng/ml), (C) remdesivir + AYUSH-64 (2001–1.001 μg/ml), (D) AYUSH-64 (2000–1 μg/ml), (E) Withania somnifera (2000–1 μg/ml), and (F) remdesivir + Withania somnifera (2001–1.001 μg/ml) on CYP3A4-mediated hydroxylation of testosterone. The graphs have been plotted for log conc. vs . percentage of inhibition.

Journal: Frontiers in Pharmacology

Article Title: In vitro effect of Withania somnifera , AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb−drug interactions in the management of COVID-19

doi: 10.3389/fphar.2022.973768

Figure Lengend Snippet: Effect of (A) ketoconazole (5.00–0.0005 μM/ml), (B) remdesivir (1,000–1 ng/ml), (C) remdesivir + AYUSH-64 (2001–1.001 μg/ml), (D) AYUSH-64 (2000–1 μg/ml), (E) Withania somnifera (2000–1 μg/ml), and (F) remdesivir + Withania somnifera (2001–1.001 μg/ml) on CYP3A4-mediated hydroxylation of testosterone. The graphs have been plotted for log conc. vs . percentage of inhibition.

Article Snippet: The concentration of case–control small-molecule remdesivir was prepared in a range of 1–1,000 ng/ml ( ).

Techniques: Inhibition

IC 50 values of Withania somnifera and AYUSH-64 extracts for CYP3A4, 2C8, and 2D6 isoenzymes.

Journal: Frontiers in Pharmacology

Article Title: In vitro effect of Withania somnifera , AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb−drug interactions in the management of COVID-19

doi: 10.3389/fphar.2022.973768

Figure Lengend Snippet: IC 50 values of Withania somnifera and AYUSH-64 extracts for CYP3A4, 2C8, and 2D6 isoenzymes.

Article Snippet: The concentration of case–control small-molecule remdesivir was prepared in a range of 1–1,000 ng/ml ( ).

Techniques:

Effect of (A) rosiglitazone (100–0.01 μM/ml), (B) remdesivir (1,000–1 ng/ml), (C) remdesivir + AYUSH-64 (2001–1.001 μg/ml), (D) AYUSH-64 (2000–1 μg/ml), (E) Withania somnifera (2000–1 μg/ml), and (F) remdesivir + Withania somnifera (2001–1.001 μg/ml) on CYP2C8-mediated hydroxylation of paclitaxel. The graphs have been plotted for log conc. vs . percentage of inhibition.

Journal: Frontiers in Pharmacology

Article Title: In vitro effect of Withania somnifera , AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb−drug interactions in the management of COVID-19

doi: 10.3389/fphar.2022.973768

Figure Lengend Snippet: Effect of (A) rosiglitazone (100–0.01 μM/ml), (B) remdesivir (1,000–1 ng/ml), (C) remdesivir + AYUSH-64 (2001–1.001 μg/ml), (D) AYUSH-64 (2000–1 μg/ml), (E) Withania somnifera (2000–1 μg/ml), and (F) remdesivir + Withania somnifera (2001–1.001 μg/ml) on CYP2C8-mediated hydroxylation of paclitaxel. The graphs have been plotted for log conc. vs . percentage of inhibition.

Article Snippet: The concentration of case–control small-molecule remdesivir was prepared in a range of 1–1,000 ng/ml ( ).

Techniques: Inhibition

Effect of (A) quinidine (100–0.01 μM/ml), (B) remdesivir (1,000–1 ng/ml), (C) remdesivir + AYUSH-64 (2001–1.001 μg/ml), (D) AYUSH-64 (2000–1 μg/ml), (E) Withania somnifera (2000–1 μg/ml), and (F) remdesivir + Withania somnifera (2001–1.001 μg/ml) on CYP2D6-mediated hydroxylation of dextromethorphan. The graphs have been plotted for log conc. vs. percentage of inhibition.

Journal: Frontiers in Pharmacology

Article Title: In vitro effect of Withania somnifera , AYUSH-64, and remdesivir on the activity of CYP-450 enzymes: Implications for possible herb−drug interactions in the management of COVID-19

doi: 10.3389/fphar.2022.973768

Figure Lengend Snippet: Effect of (A) quinidine (100–0.01 μM/ml), (B) remdesivir (1,000–1 ng/ml), (C) remdesivir + AYUSH-64 (2001–1.001 μg/ml), (D) AYUSH-64 (2000–1 μg/ml), (E) Withania somnifera (2000–1 μg/ml), and (F) remdesivir + Withania somnifera (2001–1.001 μg/ml) on CYP2D6-mediated hydroxylation of dextromethorphan. The graphs have been plotted for log conc. vs. percentage of inhibition.

Article Snippet: The concentration of case–control small-molecule remdesivir was prepared in a range of 1–1,000 ng/ml ( ).

Techniques: Inhibition